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ISHS Acta Horticulturae 227: International Symposium on Vegetative Propagation of Woody Species

FACTORS AFFECTING THE PROLIFERATION AND ROOTING OF CHESTNUT IN VITRO

Authors:   C. Piagnani, T. Eccher
Abstract:
In order to improve shoot proliferation of chestnut and to investigate the influence of nitrogen on the appearance of vitrified shoots, different N and NH4+ levels on MS modified media were compared. The highest proliferation rates and the lowest vitrification incidence were observed on media containing low total N levels and low NH4+/NO3- ratios. Shoots from embryos showed better proliferating and rooting ability compared with shoots from axillary buds of three different clones.

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