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ISHS Acta Horticulturae 227: International Symposium on Vegetative Propagation of Woody Species

REGULATION OF AXILLARY BRANCHING IN MICROPROPAGATION OF WOODY FRUIT SPECIES

Author:   Ph. DRUART
Abstract:
In conditions of variable propagation through subcultures, axillary branching can be regulated by :
  • Vitamin D2 (1–10 ml/l), enhancing the propagation rates after a few subcultures and during a number of subcultures depending on the vitamin concentration in the culture medium
  • L-methionine (50–100 mg/l), stimulating B.A.P. activity related to the concentration of the amino acid
  • Glutamic acid (50–100 mg/l), stimultaneously reducing the number and the elongation of the axillary shoots
  • Galactose (1–2%), reversing the curly and darker aspect of vitrified leaves into normal ones.

Our results also showed that the successful planning of largescale tree propagation greatly depends on the composition of the culture medium initially adopted.

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