ISHS Acta Horticulturae 227: International Symposium on Vegetative Propagation of Woody Species
REGENERATION IN VITRO OF OLEASTER (ELAEAGNUS ANGUSTIFOLIA L.) FROM SHOOT TIPS OF MATURE TREE |
| Authors: | A.S. Economou, M.J. Spanoudaki |
| Abstract:
Leaf-less shoots from the previous year's growth of a 35-year-old oleaster tree (Elaeagnus angustifolia L.) were collected in February and maintained in a warm and illuminated growth chamber for budbreak. The softwood growth which was produced after 2 weeks provided explant material for the experiments. A 3.5-fold multiplication rate was achieved from shoot tips after a 6-week culture on slightly modified WPM medium with 0.5 μM benzyladenine (BA), and 1.3-fold multiplication rate with 0.5 μM kinetin. N6-(Δ2-isopentenyl)-adenine (2iP) at concentrations 0.5, 5 and 50 μM failed to induce multiple shoot formation. During the first passage, the medium with 0.5 μM BA produced more shoots than that with 0.5 μM kinetin. The number of shoots was increased in the second passage with the addition to the medium of 0.5 μM BA. The foot half-part of in vitro-derived shoots produced more axillary shoots than the top half-part on a medium containing 0.5 μM BA. Indolebutyric acid (IBA) added to the medium at concentrations 0.5 and 5 μM had little or no effect, respectively, on microshoot rooting. IBA at 50 μM inhibited entirely microshoot rooting. Ninety five per cent rooting occurred in an auxin-free medium. Rooted microshoots were established in soil mixture under intermittent mist and grew successfully to whole plants in greenhouse conditions. | |
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