ISHS Acta Horticulturae 227: International Symposium on Vegetative Propagation of Woody Species
ADVANCES IN CHESTNUT MICROPROPAGATION (CASTANEA SP.) |
| Authors: | J.E. CHAUVIN, G. SALESSES |
| Abstract:
Vegetative multiplication of chestnut varieties is rather difficult to obtain. High levels of phenolic compounds and lack of reactivity of mature tissues are the main problems to be solved to succeed in adult material multiplication using in vitro techniques. In order to get reactive material we tried both meristem and classical axillary bud culture. The material we worked on consisted of varieties of Castanea sativa, of C. crenata and of mature hybrids C. sativa x C. crenata. As far as the percentage of buds that developed is concerned, genotypes, explant harvesting dates and conditionning of the mother-trees seem to be determinant. Leafy shoot formation from meristems occured on only one medium containing benzylaminopurine, indolebutyric acid and gibberellic acid. This type of material is compared with the one obtained from "classical" explants. The addition of ascorbic acid to the medium and the choice of the right explant (position and date) partially solved the problem with phenols. The required level of BAP varied according to genotypes. The modification of the classical carbon source and the use of "double-phase" medium proved to be the most efficient solutions for the improvement of the cultures. Two physical factors, irradiance and temperature, were studied and appeared to have a remarkable effect on chestnut micropropagation. Rooting was achieved by dipping the basal ends of shoots formed in vitro into an auxin solution. Good acclimatization was obtained in greenhouse. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
