ISHS Acta Horticulturae 227: International Symposium on Vegetative Propagation of Woody Species
ROOT INDUCTION BY AGROBACTERIUM RHIZOGENES IN A DIFFICULT-TO-ROOT WOODY SPECIES. |
| Authors: | L. Patena, E.G. Sutter, A.M. Dandekar |
| Abstract:
Notable difficulties exist in rooting cuttings of many woody species using conventional methods. We have been using Agrobacterium rhizogenes, a naturally-occurring bacterium, to induce roots in a difficult-to-root subclone of 'Golden Delicious' apple which has been maintained in vitro for over 4 years. Four strains of A. rhizogenes were tested. State of the bacteria, size and concentration of bacterial inoculant, and stage of growth of shoots were examined. Root formation occurred with 2 of the 4 strains when colonies were used as inocula. Apple shoots that produced roots continued to grow in vitro and the roots continued to elongate while in culture. These studies indicate that inoculation with A. rhizogenes may be a means of inducing adventitious root formation in cuttings of difficult-to-root woody species. | |
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