ISHS Acta Horticulturae 227: International Symposium on Vegetative Propagation of Woody Species
EFFECT OF POTASSIUM AND NITROGEN CONCENTRATION ON GROWTH OF PEACH SHOOTS CULTURED IN VITRO. |
| Authors: | F. Loreti, S. Morini, S. Concetti |
| Abstract:
Many of the problems arising in the in vitro propagation of fruit tree species are attributed largely to a defective balance of growth regulators in the culture medium, while the relative amounts of macroelements are generally less considered. However, the influence of the mineral components is not yet fully understood, so that precise statements on their importance are premature. Studies carried out to date on in vitro peach culture disagree as regards nutrient element requirements: positive results have been obtained with media having nitrogen concentrations of 60 mM as in the Murashige and Skoog medium (MS) (1962) (Skirving and Chu, 1977; Hammershlag, 1982; Almhedi, 1982; Millet et al., 1982), of around 30 mM as in the Cheng (1978) and PM (Fiorino and Leva, 1981) (Bini and Sanesi, 1982; Rodriguez, 1982; Reeves et al., 1983) and of 15 mM as in the WPM medium (Lloyd and McCown, 1980), (Morini and Concetti, 1984). Also the potassium content in these media is variable ranging from 10 to 20 mM, while the quantities of the other macroelements remain relatively constant. This study compared media with different nitrogen and potassium concentrations with the aim of identifying the most effective balance for proliferation and rooting of peach shoots. | |
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