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| Authors: | P.J. Langford, H. Wainwright |
Abstract:
Reducing the concentration of sucrose in the culture medium over successive subcultures has been tested as a method for increasing the ability of rose shoots grown in vitro (Rosa cvs.
Iceberg and Peace) to take up CO2. Shoots maintained on 'constant' 10, 20 and 40 gl-1 sucrose showed decreased levels of CO2 uptake at higher sucrose concentrations, although cv.
Peace grew least at 10 gl-1 and showed correspondingly low amounts of CO2 uptake compared with 20 and 40 gl-1. Both cultivars died when sucrose was omitted from the medium.
Vitrification increased with decreasing concentration of sucrose.
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