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ISHS Acta Horticulturae 227: International Symposium on Vegetative Propagation of Woody Species

INFLUENCE OF SUCROSE CONCENTRATION ON THE PHOTOSYNTHETIC ABILITY OF IN VITRO GROWN ROSE SHOOTS

Authors:   P.J. Langford, H. Wainwright
Abstract:
Reducing the concentration of sucrose in the culture medium over successive subcultures has been tested as a method for increasing the ability of rose shoots grown in vitro (Rosa cvs. Iceberg and Peace) to take up CO2. Shoots maintained on 'constant' 10, 20 and 40 gl-1 sucrose showed decreased levels of CO2 uptake at higher sucrose concentrations, although cv. Peace grew least at 10 gl-1 and showed correspondingly low amounts of CO2 uptake compared with 20 and 40 gl-1. Both cultivars died when sucrose was omitted from the medium. Vitrification increased with decreasing concentration of sucrose.

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