ISHS Acta Horticulturae 227: International Symposium on Vegetative Propagation of Woody Species
ANATOMICAL FEATURES OF ROOTING IN WOUNDED WINTER CUTTINGS OF THE APPLE ROOTSTOCK M.26 |
| Authors: | K.A.D. MacKenzie, B.H. Howard, R.S. Harrison-Murray |
| Abstract:
Wounding improved the rooting of winter cuttings of M.26 which were collected without the basal rosette of nodes. The internode tissue thus exposed at the base of the cuttings would normally not produce roots. Three types of wound were used: 1. Two shallow incisions, which gave no improvement, 2. Two deep incisions, which gave a 4-fold improvement, 3. Split base, which gave a 16-fold improvement. There was heavy callusing of the cutting base and wounds; this was produced mainly by the phellogen, cortex and outer phloem. An important feature of the successful treatments, however, was the damage to the cambium. In the deep incision treatment this resulted in the production of callus of cambial origin within the general mass of callus. A new cambium regenerated within the cambial callus in the form of an outward pointing salient. In the split-base treatment two such salients were formed. The salients were subdivided horizontally by the rays at the edge of the wound into finger-like projections containing xylem. Root primordia were initiated in the periphery of the callus, and after the establishment of a vascular link between the primordia and one or more projection, outgrowth of the resultant root proceeded rapidly. | |
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