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ISHS Acta Horticulturae 227: International Symposium on Vegetative Propagation of Woody Species

THE EFFECTS OF REVERSED POLARITY AND ACROPETAL CENTRIFUGATION ON THE ROOTING OF HARDWOOD CUTTINGS OF GRAPEVINE ROOTSTOCK KOBER 5BB

Authors:   G. Cristoferi, N. Filiti, F. Rossi
Abstract:
Rooting characteristics, soluble carbohydrates and the patterns of auxin-like substances produced during rhìzogenesis were determined in cuttings that were centrifuged acropetally or basipetally prior to planting and planted in an inverted or normal position.

The polarity reversal caused a drastic decrease in rooting percentage, despite high rooting being normal in this rootstock; this negative effect disappeared with IBA treatment. By contrast, acropetal centrifugation merely mitigated the negative effects of inversion on rooting percentage. Inverted cuttings began rooting later and more slowly than the normally oriented ones; however, no change in the normal histological origin of roots was observed.

Laboratory tests showed marked changes caused by reversed polarity in the total soluble carbohydrate content and in the auxin patterns at different stages of root formation.

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