ISHS Acta Horticulturae 227: International Symposium on Vegetative Propagation of Woody Species
USE OF LEAF PETIOLES OF JUVENILE AND MATURE HEDERA HELIX TO STUDY CONTROL OF ADVENTITIOUS ROOT INITIATION |
| Authors: | Wesley P. Hackett, Robert L. Geneve, M. Mokhtari |
| Abstract:
Two experimental systems for study of regulation of root initiation in easy and difficult-to-root tissue are described. One system utilizes in vitro cultured, debladed petioles of juvenile and mature leaves and the other involves approach grafting of detached leaves to form cuttings composed of the four reciprocal combinations of juvenile and mature petioles with juvenile and mature lamina. The results of experiments with these systems lead to the following conclusions: 1) the morphogenetic process of root initiation is very different in easy and difficult-to-root tissues, 2) auxin is required for root initiation in de-bladed juvenile petioles but stimulates only unorganized cell division in mature de-bladed petioles, 3) exogenously applied auxin has similar distribution patterns in juvenile and mature de-bladed petioles, 4) root formation is a function of the rooting potential of cells localized in the petiole, 5) there is no evidence of a rooting inhibitor being transported from mature lamina, and 6) a translocatable substance(s) formed in juvenile lamina can either induce root initiation in mature petioles or increase rooting potential of new cells formed as a result of auxin treatment. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
