ISHS Acta Horticulturae 227: International Symposium on Vegetative Propagation of Woody Species
THE SHOOT-TIP GRAFTING TECHNIQUE APPLIED IN CITRICULTURE. |
| Authors: | A. Starrantino, A. Caruso |
| Abstract:
The technique of micrografting is commonly applied to the recovery of Citrus clones from virus diseases. It consists of "in vitro" grafting of an excised shoot tip apex onto decapitated seedlings as rootstock. Applying this technique numerous clones of clementine, sweet orange, lemon and of other species of Citrus were recovered from all the virus diseases that they carried; at present all these clones are being grown in order to observe their production before release for commercial propagation. The percentage of the apices that survived, using the above mentioned technique, was about 40%. Tests were carried out using several growth substances and another method of micrografting to improve the survival rate. It was discovered that by dipping the apex and the decapitated seedlings for ten minutes before micrografting in a solution of 6-BAP at 0.5 ppm, the percentage rate of sprouting increased from 73% to 91%. A survival rate of 63% has been obtained through the lateral micrografting. | |
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