ISHS Acta Horticulturae 226: International Symposium on Propagation of Ornamental Plants
A NOVEL DISPOSABLE CULTURE VESSEL MADE OF FLUOROCARBON POLYMER FILMS FOR MICROPROPAGATION |
| Authors: | M. Tanaka, M. Goi, T. Higashiura |
| Abstract:
Fluorocarbon polymer films (Neoflon® films, Daikin Industries, Ltd.) possess superior properties; thermal stability, chemical inertness, light transmittance, gas-permeability and heat seal processability. Using these films, we have developed new types of culture vessels, envelope-shaped and box-shaped ones, which are found to be suitable for practical applications in protocorm-like body (PLB) proliferation of Cymbidium and in the shoot development, respectively. Two sheets of films (Neoflon FEP, 100 μm) were shaped into an envelope form (15 cm long by 8 cm wide, 4 cm top-opening) by a heat sealer, referred to as the 'Culture Bag'. 80 ml of liquid medium was poured in the autoclaved 'Culture Bag' through an opening. After one fourth segments of the PLBs derived from shoot tip culture were inoculated, the 'Culture Bag' was closed by Closure (Spectrum Medical Industries, USA) and then heat sealed. Average number of proliferated PLBs per segment in the 'Culture Bag' was larger than those on solid medium, in stationary liquid medium and in shaken liquid medium in flasks. A box-shaped culture vessel (7.5 x 7.5 x 10.5 cm), the 'Culture Pack', was also made of Neoflon films. The plantlets grown on soild medium in the 'Culture Pack' were normal, and the size of them was almost the same as those grown in flasks. There are several advantages to using these culture vessels in tissue culture; it is inexpensive, disposable, transportable, preservable and furthermore a completely closed sterile system. | |
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