ISHS Acta Horticulturae 226: International Symposium on Propagation of Ornamental Plants
IN VITRO PROPAGATION OF CYCLAMEN PERSICUM MILL. |
| Authors: | H.-G. Schwenkel, J. Grunewaldt |
| Abstract:
The vegetative propagation of Cyclamen persicum is mainly important for genetic investigations and the development of breeding systems. The vegetative propagation allows for example to test the combining ability of potential F1 hybrid parent lines extensively and to store simultaneously the genotypes during the progeny testings. Afterwards the selected parent genotypes could be multipled, starting from the in vitro stored material. This propagation and storage procedure guarantees the identical reproduction of the parents tested and selected, and it prevents genotypic alterations which will occur after the generative multiplication. In addition, the expected strong inbreeding depression after only very few generations of selfing is prevented as well as the low seed set of inbred Cyclamen lines. But also in specific tetraploid Cyclamen types, as for example the "Rokkoko" and the "eyed" flower types, in which the desired characters are expressed as gene dosage effect, the vegetative propagation allows a more extensive and efficient selection of the particular parent genotypes needed. The selected cross-parents could be stored and multiplied in vitro as described for the hybrid breeding sheme. Although a lot of investigations to multiply Cyclamen in vitro have been carried out (Fersing et al., 1982; Geier, 1977; Hoffmann and Preil, 1987) the results obtained show a lot of fundamental problems: highly contaminated explants, low regeneration rate, regeneration of single leaves without meristems at the basis, and pronounced genotypic specificity for in vitro propagation ability. | |
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