ISHS Acta Horticulturae 226: International Symposium on Propagation of Ornamental Plants
SAINTPAULIA IONANTHA H. WENDL.- IN VITRO PROPAGATION AND ACCLIMATIZATION IN A COMMERCIAL LABORATORY |
| Authors: | D. Schulze, R. Mayer |
| Abstract:
The masspropagation technique in the laboratory is a three step system. 1. Initial culture of leafsegments up to in vitro motherplant establishment. 2. recurrent propagation (one initial culture is used for 5 sets of each 10000 plantlets); 3. Shootenlargement. The media for propagation and shootenlargement are described. A comparison between two methods of acclimisation in the greenhouse ist carried out. In method A singled in vitro plants are adapted to soil. For 10.000 hardened plantlets a total working time of 101,25 hours is needed. Method B is a two step system. The african violets are transfered and soiladapted as plantclusters. After hardening plantlets are isolated. The working time amounts to 118,75 h. Although the two step system of acclimatization is more expensive it is commonly used. The uniformity of plantsize in a unit of 100 or more plantlets must be comparable to conventional by leafcuttings propagated african violets. | |
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