ISHS Acta Horticulturae 226: International Symposium on Propagation of Ornamental Plants
COMPARATIVE ANATOMICAL AND PHYSIOLOGICAL STUDIES WITH ORNAMENTAL PLANTS UNDER IN VITRO AND GREENHOUSE CONDITIONS |
| Author: | G. Reuther |
| Abstract:
Histological and physiological investigations with two cultivars of Pelargonium zonale and one cv. of Spathiphyllum floribundum and Rosa rugosa have been performed under in vitro and subsequent ex vitro conditions. Cross sections from 3 different developmental stages of in vitro leaves showed an insufficient differentiated palisade parenchyma and abnormally shaped chloroplasts. The dry matter production and chlorophyll content per fresh weight was reduced. Only leaflets in the mitotic phase fully adapted to greenhouse conditions; structural features of vitrification in older leaves remained unchanged. A small net photosynthesis was measured with rooted in vitro plants at a low light compensation point corresponding with the light and temperature conditions of the growth cabin. The CO2-fixation was stimulated in a high degree at atmospheric CO2-enrichment of 400–1000 ppm. After exposure of Pelargonium in vitro plantlets to high light intensity (from 80 μE· m-2· s-1 up to 260 μE· m-2· s-1) for 1 week, a conversion to high light character expressed in an increased saturation point and photosynthetic rate was observed. The influence of the light intensity on water vapour release could be demonstrated. The consequences for the production of improved material for transfer to soil are discussed. | |
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