ISHS Acta Horticulturae 226: International Symposium on Propagation of Ornamental Plants
AZALEA REGENERATION FROM CALLUS CULTURE |
| Authors: | A. S. Economou, Paul E. Read, Maria J. Spanoudaki |
| Abstract:
Callus formation from stem internodal pieces (2–3 mm long) of in vitro-derived shoots of azalea (Rhododendron sp. 'White Lights' and Rhododendron sp. Accession 800113) was enhanced on a low-salt medium containing 1 μM 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5–5 μM N6-(Δ2-isopentenyl)-adenine (2iP). Explant survival and callus production were better in light than in darkness. Adventitious bud formation on callus cultures occurred on medium supplemented with 0–1 μM indoleacetic acid (IAA) or naphthaleneacetic acid (NAA) and 1–10 μM 2iP. A combination of 0.1 μM IAA and 10 μM 2iP induced shoot formation on agar-based medium, whereas on liquid medium (filter-paper bridge) the plant growth regulators had no effect. Shoots produced from callus cultures rooted readily in a peat:perlite mixture (2:1 V/V) and grew into plants with phenotypes characteristic of their parents. | |
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