ISHS Acta Horticulturae 226: International Symposium on Propagation of Ornamental Plants
MANIPULATION OF DIFFERENTIATION IN SPIRAEA IN VITRO |
| Authors: | Colin R. Norton, M. E. Norton |
| Abstract:
Spiraea is a useful test plant for micropropagation due to its very rapid shoot proliferation and growth rate in vitro - as many as 100 shoots were produced from one shoot propagule in four weeks. Shoot proliferation rates were compared in standard micropropagation systems using cytokinin at high concentrations with rates in systems where the cytokinin requirement was partially relaced by changes in the gaseous environment. In standard systems, shoot proliferation rate increased over the first few generations and then declined. The extent of this decline depended on the type and concentration of cytokinin used - the decline was almost eliminated with 2iP but was very severe when BA was the cytokinin used. As the repeated dosing of the shoots with cytokinin appears to be responsible for this decline in shoot proliferation, it is proposed that reduction of cytokinin concentration and induction of shoots by alternative means could reduce such problems. Nitrogen gas or red light treatment enhanced shoot proliferation rates significantly when compared to controls and could be a useful substitute for cytokinin. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
