ISHS Acta Horticulturae 226: International Symposium on Propagation of Ornamental Plants
STOCK PLANTS INFLUENCE MICROPROPAGATION SUCCESS |
| Author: | Paul E. Read |
| Abstract:
The rate of micropropagation, as well as the quality of the resulting propagule, has been demonstrated to be markedly influenced by the condition and treatment of the stock plant (donor plant, mother plant). Some results of micropropagation are influenced in a fashion similar to the expected response when using macropropagation techniques, while in other cases the responses may be almost opposite. Light intensity, light quality and photoperiod under which the stock plant is grown can be manipulated to enhance micropropagation success. In some instances the results can be correlated with a stimulation of photosynthetic activity, which in turn produces explant material of greater regenerative capacity. Light quality can be modified to manipulate the desired result; e.g. red light applied to the stock plant of petunia and azalea has increased the number of shoots produced, suggesting a stimulation of cytokinin production, while auxin levels appear to be at higher levels when stock plants are illuminated with far red light. Selection of preferred temperatures in which hyacinth bulbs are stored can lead to enhancement of bulblet formation from scale explants cultured in vitro. Likewise, plant growth regulator and nutrient treatments of the stock plant can be applied to increase shoot proliferation and modify other in vitro responses. Forcing solutions can be used to extend explant harvest season of woody species and as a carrier of growth regulating chemicals to the softwood growth that is to be used as an explant source. | |
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