PROPAGATION OF VALLOTA PURPUREA HERB. THROUGH TISSUE CULTURE

ISHS Acta Horticulturae 226: International Symposium on Propagation of Ornamental Plants

PROPAGATION OF VALLOTA PURPUREA HERB. THROUGH TISSUE CULTURE

Authors:

K. Kukulczanka, K. Kromer

Abstract:
The purpose of this study was to examine the regeneration ability of Vallota purpurea in the tissue culture conditions. Excised double scale segments gave rise to adventitious plants after being placed on the basic Murashige and Skoog medium, which did not contain any growth hormones. In these conditions about 80% of explants formed 1 or 2 bulblets. Bulblet production on this medium decreased during subsequent subculture, thus an addition of growth hormones was necessary.

Further investigations indicated that bulb differentiation was controlled by two types of hormones which showed more individual than synergistic action, i.e.,

auxins which affected the percent of regenerating explants and growth of bulblets,
cytokinins which increased the number of bulblets per scale as well as the number of regenerating explants.

Low doses of auxin promoted root initiation at the bulblet base. Plants obtained in vitro adapted easily without any losses to the greenhouse conditions.

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