ISHS Acta Horticulturae 226: International Symposium on Propagation of Ornamental Plants
IN VITRO PROPAGATION OF IRIS HOLLANDICA TUB. CV. PROF. BLAAUW. I. REGENERATION ON BULB-SCALE EXPLANTS |
| Authors: | P.C.G. van der Linde, G.M.G.M. Hol, G.J. Blom-Barnhoorn, J. van Aartrijk, G.J. de Klerk |
| Abstract:
Bulb-scale explants of iris cultured in vitro produced adventitious shoots at the junction of the basal plate and the scales. The optimal culture condition for regeneration was 20°C in the dark on medium containing half the normal concentration of Murashige and Skoog salts. The mean number of shoots per explant was ca 10. An interruption of the 20°C-culture by a period of 4 weeks at 5°C enhanced bulb formation of the shoots. The total bulblet weight produced per single outer-scale explant was higher when the cold treatment was given at a later stage of the culture at 20°C. This was not observed for double inner-scale explants. The largest bulblets occurred on medium containing half the normal concentration of Murashige and Skoog salts. The number of bulblets produced per explant varied from 2 to 6. | |
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