ISHS Acta Horticulturae 225: Bacterial and Bacteria-like Contaminants of Plant Tissue Cultures
IDENTIFICATION OF BACTERIA BY COMPUTER-ASSISTED FATTY ACID PROFILING |
| Author: | D.E. Stead |
| Abstract:
Plant pathogenic and other plant associated bacteria obtained from the NCPPB were determined by computer-assisted fatty acid profiling. Bacteria were grown for 24 hours under defined cultural conditions. Cellular lipids were saponified, and the fatty acids were methylated, extracted and purified by a simple single tube method. The resulting fatty acid methyl esters were separated, identified and quantified by computer controlled automated gas chromatography using a software library of known fatty acid methyl esters. Profiles obtained for each of the 415 strains tested were compared with a commercially available software library of profiles of a wide range of bacteria which included all taxa tested. Accuracy of determination was approximately 98% at specific level, 72% at subspecific level but generally less than 50% at infra subspecific level, e.g. for pathovar determination. Profiles were slightly altered by changes in cultural conditions but this did not usually alter the determination. Computer-assisted fatty acid profiling offers a cheap, rapid and accurate method for determination of bacteria. | |
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