ISHS Acta Horticulturae 225: Bacterial and Bacteria-like Contaminants of Plant Tissue Cultures
THE TOXICITY TO PLANT TISSUE AND TO AGROBACTERIUM TUMEFACIENS OF SOME ANTIBIOTICS |
| Authors: | F.T. Okkels, M.G. Pedersen |
| Abstract:
Six different antibiotic compounds have been tested for their suitability in selective killing of Agrobacterium when co-cultivated together with sugar beet tissue and for their effects on callus growth and plant regeneration of carrot tissues. Cefotaxime and carbenicillin were shown to be the best suited antibiotics for selective killing of Agrobacterium when co-cultivated with sugar beet tissue, while tetracycline could be used as an alternative. Hygromycine and cefotaxime seem to enhance regeneration of plants when used at low concentrations. At higher concentrations all the antibiotics were toxic, but the high concentrations were more inhibitory to regeneration than to the callus growth. | |
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