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ISHS Acta Horticulturae 225: Bacterial and Bacteria-like Contaminants of Plant Tissue Cultures

ELIMINATION OF CONTAMINATING MICRO-ORGANISMS FROM MERISTEM CULTURE OF APPLE ROOTSTOCK M26

Author:   G.B. Poulsen
Abstract:
In meristem culture of apple rootstock M26, four contaminating organisms were isolated and cultured, i.e. two gram-negative bacteria and two yeasts.

The bacteria were eliminated by applying the cephalosporins cefotaxime and cephalosporin (50 μg/ml) to the culture medium.

Several substances were tested for their toxicity to yeast without harming the plant material, but none was found useful.

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