ISHS Acta Horticulturae 225: Bacterial and Bacteria-like Contaminants of Plant Tissue Cultures
EXPERIENCE AND PROBLEMS WITH INFECTIONS IN TISSUE CULTURE OF GRAPEVINE |
| Authors: | G. Reustle, M. Mann, C. Heintz |
| Abstract:
For the cultivation of in vitro green cuttings and meristems of grapevine a Domestos-solution as disinfective reagent was found to be cheap and effective. For satisfactory results with ovules and anthers, mercury chloride as disinfective reagent is necessary. Insect pests as vectors of secondary infections are reported. The use of a test for invisible infections has turned out to be very helpful if working with protoplasts. The antibiotics Cefotaxime and Penicillin-G were not very efficient for the elimination of bacteria. The phytotoxicity of these antibiotics could not be determined by a measurement of the respiration rate in callus cell suspensions. In vitro dual culture of powdery mildew resulted in an inoculum free of contaminants. The varietal differences in susceptibility to this pathogen with in vitro material were similar to field material, yet with an enhanced expression of the symptoms. Infection of callus tissues with Oidium tuckeri could also be achieved. Preliminary investigations on the resistance of Vitis genotypes to Agrobacterium tumefaciens, the agent of crown gall, are described. | |
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