ISHS Acta Horticulturae 218: XII African Symposium on Horticultural Crops
IN VITRO EMBRYO CULTURE OF INTERSPECIFIC HYBRIDS BETWEEN THE KENYAN LINES OF PHASEOLUS VULGARIS L. AND PHASEOLUS ACUTIFOLIUS A. GRAY |
| Authors: | S.D. Obukosia, K. Waithaka, V.K. Gupta, P.M. Kimani |
| Abstract:
The objective of the study was to examine interspecific compatibility between the Kenyan Phaseolus vulgaris lines (GLP 2, GLP 24, GLP 1004, GLP x 92 and 1122) and Phaseolus acutifolius lines (P.I. 310801, P.I. 310606, P.I. 263590, L-246–2, L-179, CIAT G40016, Buff Hood Arizona and White Hood Arizona) by in vitro embryo culture, with regard to future genetic exchange between the two species. Successful hybrids were procured between GLP24 x Buff Hood Arizona, GLP 1004 x CIAT G40016, 1122 x P.I. 310801. Rooting, which was poor among the interspecific hybrids produced in vitro, was promoted either by altering media composition or trying various genotype combination. N°-benzyladenine promoted shoot development and retarded rooting of hybrids in vitro. Exclusion of hormones promoted both shoot and root development. | |
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