ISHS Acta Horticulturae 217: IV International Workshop on Fire Blight
PRODUCTION OF RAT MONOCLONAL ANTIBODIES SPECIFIC TO ERWINIA AMYLOVORA |
| Authors: | J. Hutschemackers, M. Verhoyen, H. Bazin |
| Abstract:
Hybridoma cell lines secreting monoclonal antibodies to Erwinia amylovora were produced by fusing splenocytes or popliteal ganglionary cells from immunized LOU/C rat with myeloma cells of the nonsecreting IR983F fusion line. Three hybridoma cell lines (LO-Ea-10, LO-Ea-15 and LO-Ea-18) were selected because they produced monoclonal antibodies specific to E. amylovora. The monoclonal antibodies (LO-Ea-10, LO-Ea-15 and LO-Ea-18) purified from ascitic fluids reacted in indirect immunofluorescence and in indirect ELISA with 93 strains of E. amylovora from 7 different geographic areas. These monoclonal antibodies did not react with strains of E. mallotivora, E. quercina, E. herbicola, E. milletiae, E. carotovora, E. chrysanthemi and E. uredovora. Moreover they did not react with Pseudomonas, Xanthomonas and Enterobacter species. The monoclonal antibody LO-Ea-18 reacted by indirect ELISA with the supernatants of liquid cultures inoculated with 90 strains of E. amylovora but did not react with the supernatants of liquid cultures inoculated with 88 strains of other bacterial erwinia, corynebacteria, pseudomonads, xanthomonads, agrobacteria and enterobacteria species. The monoclonal antibody LO-Ea-18 can be used for the detection of fireblight. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
