ISHS Acta Horticulturae 217: IV International Workshop on Fire Blight
THE EFFECT OF OSMOTIC CONCENTRATION ON THE PRODUCTION OF HERBICOLACIN 112Y BY ERWINIA HERBICOLA 112Y |
| Authors: | R.S. Wodzinski, G. Stegner, S.V. Beer, P. Sobiczewski |
| Abstract:
Erwinia herbicola strain Eh112Y sometimes produces a bacteriocion, called herbicolacin 112Y (H-112Y), that inhibits the growth of Erwinia amylovora, the fire blight pathogen. Herbicolacin 112Y was not produced when Eh112Y was grown on a glucose-mineral salts medium with complex nitrogen sources. Addition of nutrient broth, yeast extract, casamino acids, or a combination of nutrient broth and yeast extract inhibited production. However, H-112Y was produced in the same media when the concentration of potassium phosphate was 0.18 M. Production of H-112Y was stimulated when the concentration of NaCl was 0.5 M or the concentration of sorbitol was 1.0 M. High osmotic concentration stimulated H-112Y production and did not simply cause the release of H-112Y that adhered to cell components. | |
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