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| Authors: | M. Wilson, H.A.S. Epton, D.C. Sigee |
Abstract:
Flowers of Hawthorn (Crataegus monogyna) were artificially inoculated with the fire blight pathogen (Erwinia amylovora) in the laboratory, and disease progression monitored under maintained conditions of high humidity at 20°C. Scanning electron microscopy revealed a rapid multiplication of bacteria at the nectary surface, mainly in and around the nectarthodes.
Light microscopy showed that although the bacteria entered the subnectarthodal chamber by 6 h, penetration of the nectary tissue did not occur until 48 h.
Control inoculation with Erwinia carotovora led to limited multiplication but no penetration of the nectary tissue.
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