ISHS Acta Horticulturae 217: IV International Workshop on Fire Blight
OBTENTION OF A STRAIN OF ERWINIA AMYLOVORA CURED OF ITS PLASMID pEA |
| Authors: | J. Laurent, M. Barny, A. Kotoujansky |
| Abstract:
All the isolates of Erwinia amylovora that have been looked at contain a cryptic plasmid called pEA which is 27.9 Kb in size. In an attempt to determine the role of this plasmid, we have undertaken experiments to obtain a strain of E. amylovora devoided of pEA. We first realized the genetical labelling of this plasmid, using the reverse genetic method deviced by Ruvkun and Ausubel (1). A fragment of pEA has been cloned in an E. coli vector and subsequently mutagenised by insertion of a Mu derivative. The labelled fragment was then introduced into E. amylovora by transformation and a strain containing only the genetically labelled pEA has been selected. Thereafter this plasmid has been eliminated by destabilization. We have not obtained a strain containing no plasmid and its properties are under studies. | |
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