ISHS Acta Horticulturae 217: IV International Workshop on Fire Blight
IN VITRO AND IN SITU DETECTION OF ERWINIA AMYLOVORA WITH MONOCLONAL ANTIBODIES |
| Authors: | C.P. Lin, T.A. Chen, J.M. Wells, T. van der Zwet |
| Abstract:
Monoclonal antibodies (MAs) specific to Erwinia amylovora were used to identify and detect epiphytic and endophytic E. amylovora in bacterial cultures and pome fruit tissues by indirect ELISA and immunfluorescent staining. Ten clones of species specific MAs did not cross-react to any epiphyte or identified bacterial strain in the ELISA. The antibodies were thus used in the in vitro identification and detection of E. amylovora in different bacterial cultures by indirect immunofluorescent staining. Except for one clone, the other nine clones reacted with the surface antigens of E. amylovora to yield strong fluorescence and were further used in the in situ detection. Positive fluorescence was observed under epifluorescent microscope in infected apple tissues but not in healthy controls. Sensitivity of the indirect biotinylated ELISA were also determined to be about 5 x 104 bacteria per test sample. | |
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