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ISHS Acta Horticulturae 216: III International Symposium on Carnation Culture

AMINO-OXYACETIC ACID: ANALYSIS AND TOXICOLOGY

Authors:   E.J. Woltering, H. Harkema, P.C.H. Hollman, M.A. Maclaine Pont
Abstract:
Pretreatment of carnation cut flowers cv. Scania with amino-oxyacetic acid (AOA) was shown to enhance the keeping quality of these flowers. Introduction of this chemical in commercial floriculture however, requires information about the toxicological aspects and a method to check whether flowers are pretreated or not. From the literature it was concluded that subcutaneous or intraperitoneal administration of AOA to small animals gives LD50 values of about 50 mg/kg. However, oral administration of relatively high doses (200–400 mg/day) for longer periods of time (weeks to months) to humans did not cause serious problems.

A high performance liquid chromatographic method was developed for the determination of AOA. The method is based on the reaction of AOA with pyridoxal to form an 0-substituted oxime, which can be determined with fluorescence detection.

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