ISHS Acta Horticulturae 212: Symposium on In Vitro Problems Related to Mass Propagation of Horticultural Plants
ORGANOGENESIS IN HYBRID COCONUT EMBRYO CULTURE |
| Author: | S. Tahardi |
| Abstract:
In vitro multiplication of coconut palms was attempted using embryo culture. Embryos from mature nuts of Bali x Nias hybrid (KINA II) were initially cultured in a half-strength Eeuweens Y3 liquid medium containing 0.1 mg/1 NAA and 2% sucrose. After 2 weeks in the liquid phase, the embryos were sliced and transferred to a full-strength Y3 agar medium containing 0.2, 1, 5 and 10 mg/1 2, 4-D, 0.25% activated charcoal and 3% sucrose. Embryos initially conditioned in the liquid medium underwent extensive tissue growth. Subsequent culture of the cotyledonary slices from the enlarged embryo on agar medium containing 5 or 10 mg/1 2,4-D stimulated nodular proliferations resembling protocorms of orchid callus. These nodules could be subcultured with reduced level of 2,4-D, eventually reverting to callus, or in combination with BAP to stimulate further nodular development suggestive of shoot organization. These nodules developed roots in the presence of NAA. | |
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