ISHS Acta Horticulturae 212: Symposium on In Vitro Problems Related to Mass Propagation of Horticultural Plants
MICROPROPAGATION OF JUVENILE CALOCEDRUS DECURRENS |
| Author: | S. Jelaska |
| Abstract:
Adventitious and axillary bud formation in incence-cedar (Calocedrus decurrens (Torr.) Florin.) embryo and seedling cultures was induced in different ways. Non callus-mediated adventitious bud induction was obtained from cotyledon of embryos or 14-day-old germinants on an agar medium WPM in the presence of 7 μM BA. Flattened cotyledons on agar medium, their connection with an apical meristem and culturing on an induction medium for more than three weeks led to a higher percentage of inducible explants and a higher number of buds per culture. Development of axilarry shoots was induced on 6–8 week-old seedling explants, on terminal shoots of 5-year-old plants, and on in-vitro-germinated shoots given a 3-h pulse treatment with high-concentration (125 μM) BA and cultured on the WPM medium supplemented with 0.025 μM NAA. Elongation growth was achieved through the subculturing of single shoots on WPM with the addition of a reduced concentration of sucrose (58.4 mM), myo-inositol (55 μM) and 0.0054–0.054 μM NAA during 2–3 subcultures, each of 21 days. Some adventitious shoots rooted spontaneously on the elongation medium, but a higher percentage of rooted shoots were obtained after the culturing of shoots for 10 days on the CD medium containing 9.8 μM IBA with 2.7 μM NAA, subsequently transferred to a sterile peat-sand-soil (1:1:1) mixture. | |
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