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ISHS Acta Horticulturae 212: Symposium on In Vitro Problems Related to Mass Propagation of Horticultural Plants

STOCK PLANT INFLUENCE ON TISSUE CULTURE SUCCESS

Authors:   P. Read, A. Economou
Abstract:
Characteristics of the stock (mother, donor) plant can have a profound influence on the performance of explant tissues cultured in vitro. Nutrient status, environmental conditions under which the stock plant is grown, and treatments applied to the stock plant can all affect in vitro culture success. Low or high nitrogen levels reduced the shoot proliferation ability of tomato leaf segments cultured in vitro and both N and K influenced tissue cultures of several Salix clones and species. Chlormequat sprays of tomato and daminozide sprays of dahlia influenced subsequant performance of explants taken from such treated stock plants. Dahlia stock plant photoperiod also affected callus and ethylene production of explants and an interaction with daminozide, treatments was also demonstrated.

Light quality and quantity was shown to modify apparent tissue hormone levels and red light applied to petunia stock plants resulted in an increase in shoot proliferation from leaf segment cultures. Furthermore, both light quanlity and quantity applied to azalea stock cultures were found to not only influence performance in vitro, but also strongly modified rootability of microcuttings. Low light levels (10–30 mol s-1m-2) caused significant increases in root number, rooting percentage and root growth.

212_15     212     212_17

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