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ISHS Acta Horticulturae 212: Symposium on In Vitro Problems Related to Mass Propagation of Horticultural Plants

ENDOGENOUS BACTERIA ELIMINATION IN ORNAMENTAL EXPLANT

Authors:   J. Fisse, A. Batlle, J. Pera
Abstract:
We examine the problem of endogenous bacterial contamination in the micropropagation of Syngonium podophyllum, Philodendron "Red Emerald" and Ficur elastica var decora. A bacteria that constantly appeared in the 3 cultures was isolated and cultured in a glucose medium. Antibiogrammes were done which led to the choice of 2 antibiotics: Cephaloridine and streptomycin. These were applied to the explants in a series of trials which allowed us to select one of them and finally through curves of activity and phytotoxicity the adequate dose for each species was ascertained.

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