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ISHS Acta Horticulturae 208: VI International Symposium on Medicinal and Aromatic Plants, XXII IHC

MASS PROPAGATION AND GENETIC VARIABILITY OF TWO CARDENOLIDE PLANTS IN VITRO

Authors:   M. Dasgupta, T.K. Pramanik, S.K. Datta
Abstract:
Asclepias curassavica (Asclepiadaceae) and Thevetia peruviana (Apocynaceae) have been known to contain a few important therapeutic cardiac glycosides besides their ornamental value. In vitro mass propagation of these plants have been successfully established on MS and B5 media with Auxin-Cytokinin combinations. Genetic variability found in callus cultures showed variation in morphogenetic responses of regenerants. Such variations are more likely due to genetic fitness of different aneuploids grown in a particular medium supplemented with suitable hormonal balance. Organ induced chemodifferentiation of cardenolides have also been emphasised.

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