ISHS Acta Horticulturae 207: Naturally Occuring Toxins in Horticultural Food Crops, XXII IHC
THE REGULATION OF EXPRESSION OF PROTEINASE INHIBITOR GENES IN FOOD CROPS |
| Authors: | C. A. Ryan, G. Pearce, G. An, R. Thornburg |
| Abstract:
Proteinase inhibitor proteins are considered to be part of the diverse array of defensive chemicals that help protect plants from predators. The genes that code for these inhibitors are under highly complex and tissue specific regulation. Several proteinase Inhibitor I and II genes have been isolated and characterized from both tomato and potato gene libraries. The 5' and 3' regions of a wound-inducible potato Inhibitor II gene have been fused with the open reading frame of a bacterial chloramphenicol acetyltransferase (CAT) gene to provide a sensitive reporter of wound-inducibility. Tobacco plants, transformed with the fused gene, express CAT activity in response to wounding. The regulatory sequences of the potato Inhibitor II gene are being analyzed to determine the basis of wound-inducibility. | |
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