ISHS Acta Horticulturae 193: XIII International Symposium on Fruit Tree Virus Diseases
DETECTION OF STRAINS OF APPLE CHLOROTIC LEAFSPOT VIRUS BY F(ab)2-BASED INDIRECT ELISA |
| Authors: | M. Barba, M.F. Clark |
| Abstract:
A F(ab')2-based indirect ELISA was used to investigate 1) the feasibility of assaying apple chlorotic leafspot virus in fruit trees throughout the growing season, 2) the distribution of detectable antigen in aerial parts of the tree, 3) the occurrence of different serotypes of virus and the potential for discriminating among serotypes. Virus was detected in infected plum and peach early in the season, in apple both early and late in the season, but never in pear. Virus was erratically distributed with leaves toward the base of each branch more often containing virus than those toward the tip. Bark stripped from one or two year-old wood was the most reliable tissue for assay, particularly later in the growing season. It was possible to discriminate among virus serotypes by testing each isolate with two distinct antisera, one with specificity for apple isolates and one with broad spectrum specificity. Naturally-infected apple trees appeared to be infected only by apple serotypes whereas at least two serotypes were detected from plum. | |
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