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ISHS Acta Horticulturae 193: XIII International Symposium on Fruit Tree Virus Diseases

USE OF DIFFERENT METHODS TO DETECT LITTLE CHERRY DISEASE IN ORNAMENTAL CHERRY TREES

Authors:   G. Legrand, M. Verhoyen
Abstract:
Field indexing, electron or U.V. light-microscopy and double-stranded RNA isolation were used to detect little cherry disease in ornamental cherry trees. Tests were carried out to determine the best sampling sites on the shoots for light-microscopy observations and for dsRNA isolation. According to the field indexing results, light-microscopy was chosen for its sensitivity and its feasibility. This method is proposed as a reliable means for identifying cherry trees that are infected with little cherry disease.

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