BIOLOGICAL TECHNIQUES USED FOR THE STUDY OF NEW FRUIT VIRUS DISEASES

ISHS Acta Horticulturae 193: XIII International Symposium on Fruit Tree Virus Diseases

BIOLOGICAL TECHNIQUES USED FOR THE STUDY OF NEW FRUIT VIRUS DISEASES

Authors:

R. Boyé, J.C. Desvignes

Abstract:
The sanitary examination (greenhouse and nursery indexing) of stone fruit cultivars, sometimes allows to express new symptoms that seem to be caused by infectious agents like viruses.

To determine these new diseases, to characterize their causal agent, to divide possible complexes, the interest of some simple biological techniques, tested on greenhouse grown GF 305 peach seedlings, has been studied.

The inoculation was made either with plant fragment :

minimum contact time in graft transmission,
comparative indexing by limb or phloem chips,
cross-protection between strains of the same virus,

or with sap inoculum :

sap injection with a syringe, in the young shoot,
sap soaking blotting paper set under the bark.

These techniques allow to characterize some unidentified diseases such as -peach latent mosaic (PLMV), mottle leaf (MLV), butteratura, - and probably new diseases- cherry necrotic crook, peach gummosis, stocky prune -, comparatively to well identified diseases- ILAR and NEPO viruses, chlorotic leaf spot v., plum pox v., peach yellows (MLO) -

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