ISHS Acta Horticulturae 193: XIII International Symposium on Fruit Tree Virus Diseases
INTERACTION OF TIME, TEMPERATURE, AND EXPOSURE TO AIR ON INFECTIVITY OF THE PRUNE DWARF VIRUS IN UNTREATED SQUASH SAP |
| Author: | Paul R. Fridlund |
| Abstract:
Preparations of the prune dwarf virus (PDV) in untreated squash sap increased in infectivity during the first hour of aging at temperatures from -12 to 46 C when air was excluded mechanically from the inoculum. Sap exposed to air from 5 to 42 C showed a similar increase. Inocula exposed to air lost infectivity between the first and fourth hour of aging. This loss progressively accelerated when temperatures increased from 5 to 46 C. Mechanical exclusion of air from inocula prevented detectable loss in infectivity during a 4 hr aging at temperatures from 5 to 38 C, but after the first hour of aging at higher temperatures, loss in infectivity was rapid and increased as temperatures approached or exceeded the reported lower thermal inactivation point of PDV (44 C). Freezing the sap at -12 C inactivated PDV within 3 hr. | |
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