ISHS Acta Horticulturae 193: XIII International Symposium on Fruit Tree Virus Diseases
IMPROVED METHODS FOR TREE FRUIT VIRUS DETECTION |
| Authors: | A.J. Hansen, R. Hamilton, R. Martin, R. Stace-Smith |
| Abstract:
Routine virus detection and identification methods are needed for epidemiological studies, for comparisons of viruses, for quarantine and certification purposes and as a follow-up to virus therapy. During the last decades, specific methods have been developed for most tree fruit viruses and non-sap-transmissible virus-like agents (VLAs). The discussions at the previous symposia have led to a certain degree of international standardization of these methods. Recent innovations and discoveries in serology, biochemistry and basic virology are leading to the development of more specific and more sensitive detection techniques. This emerging diversity of methods will greatly benefit research work, and at the same time require international standardization needed for applied purposes such as certification and plant quarantine work. We wish to discuss the principles which should guide the development and application of these methods, as well as the anticipated time frame for their introduction. We shall evaluate methods in light of the four criteria which determine the choice in any given case namely the purpose of the work, the characteristics of the virus or VLA, the volume to be handled and the facilities available. | |
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