ISHS Acta Horticulturae 193: XIII International Symposium on Fruit Tree Virus Diseases
INVESTIGATIONS ON PLUM POX (SHARKA) VIRUS IN SPAIN |
| Authors: | G. Llacer, M. Cambra, A. Lavina, J. Aramburu |
| Abstract:
In June 1984, plum pox (sharka) virus (PPV) was detected for the first time in Spain. Initially the virus was detected by the enzyme-linked immunosorbent assay (ELISA) in Japanese plum trees, cv. Red Beaut, showing typical symptoms of the disease. The detection was later confirmed by graft-transmission on GF-305 peach seedlings and also by immunoelectron microscopy. At present our work aims at establishing the most suitable conditions for detecting PPV by ELISA, obtaining monoclonal antibodies from semi-purified solutions of PPV and determining the real incidence of the virus in Spain. The state of our work is discussed in the present communication. | |
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