ISHS Acta Horticulturae 191: Symposium on Protected Cultivation of Solanacea in Mild Winter Climates
HYBRIDIZATIONS BY PROTOPLASTS FUSIONS IN SOLANACEA |
| Authors: | A. Hassanpour-Estahbanati, C. Turpin, Y. Demarly |
| Abstract:
Our aim in the experiments reported here was to transfer the interest characters from Solanum pennellii (drought and salinity resistance) and Nicotiana tabacum (cytoplasmic male sterility) to cultivated tomato (Lycopersicon esculentum). Protoplasts of L. esculentum var. 2NI and N. tabacum were obtained by one-step enzymatic method. S. pennellii protoplasts were isolated as described by Hassanpour and Demarly (1985). Fusion of mesophyll protoplasts of L. esculentum with protoplasts of either N. tabacum or S. pennellii was induced by a solution containing 40% Polyethylene glycol. Cell wall regeneration followed by cell division were observed between 4 to 8 days after fusion, in the corresponding protoplast-culture media. Micro-calli underwent continued division with formation of green callus. Organogenesis from protoplast derived callus was achieved when green calli was transferred into regenerating media containing zeatin and GA3, in the case of S. pennellii x L. esculentum, or BAP and IAA, in the case of N. tabacum x L. esculentum. 73 plants from fusion between N. tabacum x L. esculentum and 6 plants of S. pennellii x L. esculentum protoplasts hybridization were obtained. Cytological and morphological analysis demonstrated that 4 plants from 73 regenerated plants of N. tabacum x L. esculentum and all of the 6 plants obtained between S. pennellii x L. esculentum are hybrids. | |
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