ISHS Acta Horticulturae 189: I International Symposium of the Research and Cultivation of Roses
IN VITRO PROPAGATION OF SOME ROSE CULTIVARS |
| Authors: | P. Curir, C. Damiano, T. Cosmi |
| Abstract:
Roses started to be propagated by "in vitro" culture at the Istituto Sperimentale per la Floricoltura in 1982. So far, several cultivars have been tested for the mass propagation ability. Differences have been evidentiated among the cultivars regard to the establishment in aseptic culture of primary explant and multiplication rate. Five cultivars, Bellona, Bingo, Candia, Cocktail 80 and Sonia were rooted and successfully transferred to the greenhouse. Modifications of Murashige and Skoog formula (1962) were used as media. Three days of culture in presence of activated charcoal, followed by transfer to the fresh basal medium, were found highly effective to enhance the growth of primary explant. Thiamine 2 ppm and myoinositol 200 ppm promoted the growth of unrooted explants. IAA 1 ppm and reduced amount of sucrose permitted to obtain a good percentage of rooting. | |
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