ISHS Acta Horticulturae 179: V International Symposium on Growth Regulators in Fruit Production
DORMANCY OF SOUR CHERRY PLANTLETS PROPAGATED BY IN VITRO CULTURE TECHNIQUE |
| Author: | B. Borkowska |
| Abstract:
The sour cherry cultivars, Schattenmorelle and North Star were propagated by an in vitro technique. The rooted plants taken out of the test tubes and planted in pots grew only for 1 to 2 months and were too small and not ready for planting in nursery or orchard. Efforts were made to prolong the period of active growth of plantlets by chilling the plant material and supplying growth regulators to the culture medium. The cultures, before and after placing them on the rooting medium as well as rooted plants growing in pots, were kept in cold room (4°) for up to 14 weeks. The efficiency of rhizogenesis, the final height of trees and number of side branches were then assessed. The best morphological results were obtained by chilling cultured shoots 6–8 and 4–6 weeks for Schattenmorelle and North Star, respectively. The trees obtained from these treatments were the highest and most vigorous. Prolongation of chilling beyond 8 weeks gave negative results, i.e. the plants were weak, small, and adaptation to non-sterile conditions was difficult. The chilling effect appeared only when rhizogenesis was completed and plantlets were moved to pots (the post-chilling effect). The non-chilled plants and those chilled only for 2 weeks ceased growth soon after potting and were only 10 to 15 cm high. Optimal chilling period for potted plants was similar as for cultured shoots. | |
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