ISHS Acta Horticulturae 177: IV International Symposium on Flower Bulbs
IN VITRO PRODUCTION OF BOTRYOTINIA POLYBLASTIS APOTHECIA AND FACTORS AFFECTING GERMINATION OF ASCOSPORES |
| Author: | Gary A. Chastagner |
| Abstract:
Apothecia of Botryotinia polyblastis (Gregory) Buchw. formed after 28 days when spermatized sclerotia were incubated under 14 hour photoperiods (4435 lux) at 15 C. Reciprocal matings of 40 isolates yielded apothecia in 180 out of 1,600 matings. One of 35 single ascospore isolates that produced apothecia appeared to be homothallic; the rest were heterothallic. The average size of asci and ascospores produced in vitro was: 196.7 x 10.3 μ and 15.1 x 7.6 μ, respectively. Germination of ascospores varied depending on apothecial sources. The optimum temperature range for germination was 15–20 C, and the rate of germination was the same on water agar and potato dextrose agar. | |
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