ISHS Acta Horticulturae 177: IV International Symposium on Flower Bulbs
MICROPROPAGATION OF TULIP: BULBING OF SHOOTS IN CULTURE |
| Authors: | P.G. Alderson, A.G. Taeb, R.D. Rice |
| Abstract:
Adventitious shoots arising from the outer layer of cells of floral stem explants of tulip cultivar Merry Widow, cultured on Murashige and Skoog medium containing 1 mg/l of both naphthalene acetic acid (NAA) and benzylaminopurine (BAP), are capable of initiating a bulb primordium at their base after incubation at 20°C for 12 to 16 weeks. Development of this primordium was enhanced when cultures were transferred onto media containing 1 mg/l NAA with or without 0.1 mg/l BAP. Incubation at 4°C for 12 weeks followed by a period at 25°C on media containing 6% sucrose also enhanced bulb formation at the base of shoots. Bulbing of shoots was enhanced in cultures incubated in light with a low red/far-red ratio (3.4) and after treatment with ethylene at 1 and 10 ppm applied as a gas to the culture vessels. Shoot cultures treated at 12 weeks of age showed the greatest response. Roots were induced on bulbs produced in vitro by manipulation of plant growth regulators and sucrose in the culture medium. | |
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