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ISHS Acta Horticulturae 177: IV International Symposium on Flower Bulbs

MULTIPLICATION OF TULIP BULB BY TISSUE CULTURE IN VITRO

Author:   Y. Nishiuchi
Abstract:
This study concerns on the formation of in vitro cultured scale segments derived from the bulbs of Tulipa gesneriana cv. Apeldoorn, which have been stored at high temperature after lifting, and also attempt to examine the effect of growth regulators on bulb formation of their cultured shoots. A considerable increase in the bulb forming rate was resulted from the curing treatment of their mother bulbs for 1 – 2 weeks at 30° and 35°C since two weeks after lifting, and an increase in the number of formed bulblet and an enhancement of their thickening growth were also ascertained.

Subjecting to some growth regulators only for both the former and the latter three weeks of the culture period, the bulb formation was enhanced. The number of bulblets increased with the application of 10-5 Mol of abscisic acid (ABA) at the former period and with 10-4 Mol of 2-bromoethyltrimethylammonium bromide (BCB) at the latter period during chilling. On the other hand, the bulb formation was drastically inhibited by 2-thiouracil at the former period during chilling treatment. After the chilling treatment, an application of ABA at 10-4 Mol inhibited the bulb formation of the shoots, whereas 10-4 Mol of BCB increased. The number of formed bulblets increased by 200 mg/L GA, showing longitudinal elongated bulblets. The thickening growth of the bulblets was enhanced only by capric acid.

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