ISHS Acta Horticulturae 177: IV International Symposium on Flower Bulbs
INVESTIGATING RESISTANCE IN TULIP TO TULIP BREAKING VIRUS AND TO ITS TRANSMISSION |
| Authors: | Louise R. Romanow, J.P. van Eijk, W. Eikelboom |
| Abstract:
Tulip breaking virus (TBV) can seriously depress tulip production where infection levels are high. Cultural control of TBV spread is expensive and not completely effective. An alternative control strategy is growing varieties resistant to TBV spread. A program for breeding resistant varieties relies on good screening techniques. Screening methods should provide results which agree with findings under normal field conditions. Plants should be in a stage with susceptibility comparable to that in the field at the time of TBV spread. Mechanical inoculations in the glasshouse provide a quick, uniform treatment of all plants tested and can provide information on plant/virus interactions such as resistance to virus infection and multiplication, but may not detect other important resistance factors. Aphid inoculations in the laboratory must use relevant species as vectors. They provide more realistic inoculation and allow detection of other relevant resistance factors, but are very time-consuming. Virus detection techniques such as enzyme-linked immunosorbent assay (ELISA) can measure virus establishment, replication, and movement rates. Prevention or reduction of TBV spread can be based on a number of resistance mechanisms. The appropriateness of using any resistance factor in a breeding program depends upon its variability, the ease with which it is tested, and with which it can be transferred to other varieties. | |
Download Adobe Acrobat Reader (free software to read PDF files) | |
